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KMID : 1150720140030010025
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Integrative Medicine Research
2014 Volume.3 No. 1 p.25 ~ p.33
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Physiological effects of formulation containing tannase-converted green tea extract on skin care: physical stability, collagenase, elastase, and tyrosinase activities
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Hong Yang-Hee
Jung Eun-Young
Noh Dong-Ouk
Suh Hyung-Joo
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Abstract
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Background: Green tea contains numerous polyphenols, which have health-promoting effects. The purpose of this study was to evaluate the effect of tannase-converted green tea extract (TGE) formulation on the physical stability and activities of skin-related enzymes.
Methods: Physical stability was evaluated by measuring the pH, precipitation, and colors at 25 ± 2 °C/ambient humidity and at 40 ± 2 °C/70% ± 5% relative humidity for 4 months. Activities of collagenase, elastase, and tyrosinase as skin-related enzymes were assessed on TGE formulation.
Results: The concentrations of epigallocatechin-3-gallate and epicatechin-3-gallate in green tea extract were greatly decreased to the extent of negligible level when treated with tannase. The formulation containing 5% tannase-converted green tea extract showed relatively stable pH, precipitation, and color features for 16 weeks. When TGE was added to the formulation, there was a significant increase in the inhibition of elastase and tyrosinase activities (p < 0.05) compared with the formulation containing 5% normal green tea extract.
Conclusion: The TGE could be used in cosmetics as skin antiwrinkling or depigmenting agent.
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KEYWORD
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elastase, green tea, stability, tannase, tyrosinase
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